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唐堂_2018_Nature Cell Biology

發佈日期:2018-04-02

唐堂老師為本會會員(2014起)、第一及二屆理事及現任監事,任職於中央研究院生物醫學研究所,其研究專注於,中心粒複製, 神經幹細胞,與癌細胞分裂之研究。唐老師實驗室最近於Nature Biology發表了一篇有關Myosin-Va參與絨毛生成的重要論文。感謝唐老師跟我們分享其成果如下:

Myosin-Va is required for preciliary vesicle transportation to the mother centriole during ciliogenesis

 

Nature Cell Biology, Jan 15, 2018

Primary cilia play essential roles in signal transduction and development. The docking of preciliary vesicles at the distal appendages of a mother centriole is an initial/critical step of ciliogenesis, but the mechanisms are unclear. Here, we demonstrate that myosin-Va mediates the transportation of preciliary vesicles to the mother centriole and reveal the underlying mechanism. We also show that the myosin-Va-mediated transportation of preciliary vesicles is the earliest event that defines the onset of ciliogenesis. Depletion of myosin-Va significantly inhibits the attachment of preciliary vesicles to the distal appendages of the mother centriole and decreases cilia assembly. Myosin-Va functions upstream of EHD1- and Rab11-mediated ciliary vesicle formation. Importantly, dynein mediates myosin-Va-associated preciliary vesicle transportation to the pericentrosomal region along microtubules, while myosin-Va mediates preciliary vesicle transportation from the pericentrosomal region to the distal appendages of the mother centriole via the Arp2/3-associated branched actin network.

 

原始文章:https://www.nature.com/articles/s41556-017-0018-7

唐堂老師實驗室網頁: http://www.ibms.sinica.edu.tw/tang-tang/